polyclonal rabbit primary antibody against trpv1  (Alomone Labs)

Journal: Journal of Neuroinflammation

Article Title: Motoneuronal inflammasome activation triggers excessive neuroinflammation and impedes regeneration after sciatic nerve injury

doi: 10.1186/s12974-022-02427-9

Figure Lengend Snippet: Primary and secondary antibodies used for western blot (WB), immunohistochemistry (IHC) and immunofluorescence (IF)

Article Snippet: TRPV1 (IF) , Polyclonal rabbit against TRPV1, 1:500 (Cat# ACC-030, RRID:AB_2313819, Alomone Labs, Jerusalem, Israel) , Alexa Fluor® 488 AffiniPure donkey anti-rabbit IgG (H + L), 1:500 (Cat# 711-545-152, RRID:AB_2313584, Jackson ImmunoResearch).

Techniques: Western Blot, Immunohistochemistry, Immunofluorescence, Plasmid Preparation, Recombinant

Journal: Cells

Article Title: TRPV1-Like Immunoreactivity in the Human Locus K, a Distinct Subregion of the Cuneate Nucleus

doi: 10.3390/cells7070072

Figure Lengend Snippet: Full-term newborn, case 6. Left side dorsal quadrant in two consecutive sections of the caudal medulla oblongata immunostained for TRPV1 ( a , b ) and for CGRP followed by myelin Black Gold II counterstaining ( c , d ). Strongly TRPV1- and CGRP-like immunoreactive areas, along the dorsal border of the cuneate nucleus (Cu), are located in the Locus K (box in ( a , c )) and are shown at higher magnification in ( b , d ), respectively. Gr, gracile nucleus. Scale bar: ( a ) = ( c ): 250 µm; ( b ) = ( d ): 50 µm.

Article Snippet: Rabbit polyclonal antibodies against TRPV1 (Thermo Scientific, Waltham, MA, USA), diluted 1:500, and against CGRP (Chemicon, Temecula, CA, USA), diluted 1:1000, and a guinea-pig polyclonal antibody against SP (AbCam, Cambridge, UK), diluted 1:1200, were used as primary antibody.

Techniques:

Journal: Cells

Article Title: TRPV1-Like Immunoreactivity in the Human Locus K, a Distinct Subregion of the Cuneate Nucleus

doi: 10.3390/cells7070072

Figure Lengend Snippet: Full-term newborn, case 6. ( a – c ): right dorsal quadrant of three consecutive sections of the caudal medulla oblongata immunostained for TRPV1 ( a ) and SP ( b ), and stained for myelin with Black Gold II ( c ). TRPV1-LI (boxes in ( a )) and SP-LI (boxes in ( b )) are codistributed in a parallel way in the Locus K (LK) and in the spinal trigeminal nucleus (Sp5C) substantia gelatinosa. cc, central canal; Cu, cuneate nucleus; Gr, gracile nucleus. Scale bar: ( a , b ) = ( c ) 250 μm.

Article Snippet: Rabbit polyclonal antibodies against TRPV1 (Thermo Scientific, Waltham, MA, USA), diluted 1:500, and against CGRP (Chemicon, Temecula, CA, USA), diluted 1:1000, and a guinea-pig polyclonal antibody against SP (AbCam, Cambridge, UK), diluted 1:1200, were used as primary antibody.

Techniques: Staining

Journal: Cells

Article Title: TRPV1-Like Immunoreactivity in the Human Locus K, a Distinct Subregion of the Cuneate Nucleus

doi: 10.3390/cells7070072

Figure Lengend Snippet: Adult, case 9. Right dorsal quadrant of caudal medulla oblongata immunostained for TRPV1 and counterstained with Klüver–Barrera ( a ). Locus K (LK) containing TRPV1-LI (box in ( a )) is shown in ( b ) at higher magnification. c : thin fibre bundles in the dorsolateral fasciculus cuneatus (cu). cc, central canal; Cu, cuneate nucleus; Gr, gracile nucleus; Sp5C, caudal spinal trigeminal nucleus. Scale bar: ( a ) 250 μm; ( b , c ) 20 μm.

Article Snippet: Rabbit polyclonal antibodies against TRPV1 (Thermo Scientific, Waltham, MA, USA), diluted 1:500, and against CGRP (Chemicon, Temecula, CA, USA), diluted 1:1000, and a guinea-pig polyclonal antibody against SP (AbCam, Cambridge, UK), diluted 1:1200, were used as primary antibody.

Techniques: